Protein_Domain

Part:BBa_K4161603:Design

Designed by: Zhujun Yao 姚竹君   Group: iGEM22_DKU   (2022-09-27)


AcmA3b


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 343
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 343
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 343
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 343
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I need to confirm whether I need a repetitive domain for surface protein to function and whether it is added at N or C terminus. There are many surface display systems in L.lactis, and the LysM system is tested in displaying anti-TNF-alpha nanobodies. As Visweswaran et al. recently reviewed, the peptidoglycan binding properties have been extensively utilized for surface display. Therefore, using a repetitive LysM system is likely to be successful.


Source

The sequences come from L.lactis NZ9000 genomic DNA.

References